METHOD FOR DETECTING FACTOR OF KAWASAKI DISEASE

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting a factor of Kawasaki disease detectable at the time of the outbreak of the disease and useful for an its early stage diagnosis, etc., of the disease by indicating an increase in the number of Vβ6.5-positive T-cell or the increase in the number of Vβ6.5- and Vβ2.1-positive T-cells as an index. SOLUTION: This detection method comprises indicating an increase in the number of Vβ6.5-positive T-cell or the increase in the number of Vβ6.5- and Vβ2.1-positive T-cells as an index. The gene cluster of TCRV region in peripheral blood from a patient doubted to have Kawasaki disease is amplified by an adapter-additional PCR method and the frequency of appearance of TCRVβsubfamily is detected by a reverse dot-blot method using an oligonucleotide probe specific for the TCRV subfamily containing an oligonucleotide probe shown by base sequence such as formula I and formula II. Kawasaki disease is early diagnosed by detecting a factor of the disease based on an index that the frequency of appearance of TCRVβ6.5 subfamily or the frequency of appearance of TCRVβ6.5 and TCRVβ2.1 subfamilies is significantly higher than that of the other subfamily.