AN IMPROVED METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS

摘要

The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in a n attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 producti on with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purifi ed virus of the same quality as preparations from double CsC1 banding using an ultracentrifuge.