GENE NETWORKS FOR CONTROL OF GENE EXPRESSION

摘要

The present invention relates to compositions for regulating gene expression in a cell. In particular, the invention provides composition constructs which switch expression of a gene of interest between stable "on" or "off" states in response to a transiently applied agent. The invention further provides adjustable-threshold switch constructs for expressing a gene of interest in response to the sustained application of an agent at a concentration above or below a desired threshold concentration. The invention also provides multi-state oscillator constructs in which expression of a gene of interest is periodically altered in the absence of administration of agents which are extraneous to the construct. Also provided are methods for using the invention's constructs.