Polypeptide fragments of factor VIIIC, corresponding probes and DNA sequences and DNA constructs

摘要

The present invention relates to a process for the synthesis of polypeptide fragments of human Factor VIIIC, particularly a fragment having a molecular weight of 77/80 kd, DNA sequences encoding portions of this polypeptide fragment, extrachromosomal elements comprising these DNA sequences, a complex probe for screening DNA sequences, corresponding DNA constructs, and monoclonal antibodies prepared with an immunogen comprising the above polypeptide fragments. The 77/80 kd fragment may be used for preparing a protein composition comprising a calcium bridged complex of the 77/80 kd polypeptide fragment with a 92.5 kd fragment, exhibiting a coagulation activity similar to that of human Factor VIIIC.