| 摘要 |
- New polynucleotides and polypeptides of the aro (5-enolpyruvylshikimate-3-phosphotate synthase) family (aroA) (79.7% similarity, 68.2% identity with Lactococcus lactis aroA), particulary from Streptococcus pneumoniae 0100993 (available from NCIMB 40794). - Independent claims are included for the following: (1) a nucleic acid (Ia) encoding (I), a polypeptide with a sequence of 427 amino acids given in the specification; (2) a nucleic acid (IIa) encoding a mature polypeptide (II) expressed by the aroA gene from 0100993; (3) a nucleic acid of 1284 base pairs (bp) (given in the specification), (IIIa); (4) a nucleic acid complementary to (Ia), (IIa), or (IIIa); (5) vectors containing (Ia), (IIa), (IIIa) or a nucleic acid of (4); (6) host cells containing the vectors of (5); ACTIVITY - Antibacterial MECHANISM OF ACTION - The aroA product converts shikimate-3-phosphate to 5-enolpyruvoylshikimate-3-phosphate. Inhibition of this reaction prevents the synthesis of aromatic amino acids, p-aminobenzoate acid (a precursor of folate) and ubiquinone. |
