发明名称 Stabilising medium for alphagst in urine for use in an enzyme immunoassay
摘要 PCT No. PCT/IE94/00050 Sec. 371 Date Apr. 17, 1997 Sec. 102(e) Date Apr. 17, 1997 PCT Filed Oct. 17, 1994 PCT Pub. No. WO96/12191 PCT Pub. Date Apr. 25, 1996Stabilizing medium for urinary alpha GST contains a stabilizing amount of a non-enzyne protein, such as a mixture of equal amounts (w/v) of bovine serum albumin and gelatin hydrolysate, chelating agent and a buffer, such that the medium has a pH in the range 7.0-7.5, the medium being effective to prevent loss of alpha GST immunological activity. The stabilizing medium can be used to store urine samples at temperatures of the order of -20 DEG C. without any loss of alpha GST immunoreactivity of the type observed in samples which are stored without such a stabilizing medium. The stabilizing medium also improves the immunoreactivity of alpha GST when added to fresh urine which is stored temporarily at 2-8 DEG C. prior to assay for up to two hours.
申请公布号 US6071706(A) 申请公布日期 2000.06.06
申请号 US19970817696 申请日期 1997.04.17
申请人 BIOTRIN INTELLECTUAL PROPERTIES LIMITED 发明人 DOYLE, JOHN MARTIN;KILTY, CORMAC GERARD
分类号 G01N33/531;C07K16/40;C12N9/10;C12N9/96;C12Q1/28;C12Q1/48;G01N33/53;G01N33/573;G01N33/68;(IPC1-7):G01N33/53 主分类号 G01N33/531
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