发明名称 |
Bioprocesses for preparing 7-ACA and 7-ADAC |
摘要 |
A bioprocess for preparing adipoyl-7-ACA comprising the steps: (a) transforming cells of a strain of Penicillium chrysogenum which produces isopenicillin N with an expression vector containing DNA encoding an enzyme, having expandase activity capable of accepting adipoyl 6-APA as a substrate, an enzyme having hydroxylase activity capable of accepting adipoyl-7-ADCA as a substrate and an enzyme having acetyl transferase activity capable of accepting adipoyl 7-ADAC as a substrate; (b) culturing the transformed cells from step a) in a suitable culture medium containing an adipate feedstock, wherein said cells produce adipoyl 6-APA; and (c) culturing the transformed cells producing adipoyl 6-APA of step b) under conditions suitable for expression of said DNA encoding enzyme, thereby producing the end product adipoyl-7-ACA.
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申请公布号 |
US6071713(A) |
申请公布日期 |
2000.06.06 |
申请号 |
US19990340781 |
申请日期 |
1999.06.28 |
申请人 |
GIST-BROCADES B.V. |
发明人 |
CONDER, MICHAEL J.;RAMBOSEK, JOHN A.;MCADA, PHYLLIS C.;REEVES, CHRISTOPHER D. |
分类号 |
C12N1/19;C07D501/20;C12N1/15;C12N9/00;C12N9/02;C12N9/10;C12N15/09;C12N15/52;C12N15/54;C12P;C12P35/00;C12P35/06;C12P37/04;C12R1/82;(IPC1-7):C12P35/00;C12P35/02;C12N1/14;C12N1/20 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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