| 摘要 |
The present invention relates to a method for producing antibodies reacting specifically with a polypeptide that only recognises the coding nucleic acid, wherein said method comprises the following steps: a) the DNA coding the polypeptide is expressed in a host cell using a vector having at least one sequence coding a detection signal, and the expressed polypeptide is bound to a solid phase using the detection signal; b) regardless of step a), the DNA coding the polypeptide is introduced directly into an animal, which causes the expression of the polypeptide in the animal thus inducing the formation of antibodies acting against the polypeptide; and c), the polypeptide produced in step a) is used for reacting the antibodies formed in step b), for detecting them or for enriching them. |
