| 摘要 |
An oligonucleotide inhibitor of 5'-3'exonuclease activity of thermostable polymerases, the oligonucleotide having a first structural domain (stem I), a second structural domain (loop), and a third structural domain (stem II), wherein the third structural domain (stem II) is consisting of oligonucleotides having a nucleotide sequence which is complementary to the oligonucleotide sequence of the first structural domain (stem I), the first and third structural domain are forming a first double stranded structural element (stem) by hybridization of the complementary oligonucleotide sequences at ambient temperature, the second structural domain (loop) comprising a structural element NN'XY, wherein N and N' is a nucleotide residue of the pyrimidine type or N is a nucleotide residue of the purine type and N' is a nucleotide residue of the pyrimidine type, X is a structural element of nucleotide residues within the loop formed by a turn portion in the loop and a double stranded portion, wherein the double stranded portion is consisting of two to five nucleotide residues of any kind, and the turn portion is consisting of one to four nucleotide residues of any kind, Y is a structural element consisting of up to 4 nucleotide nucleotides of any kind. |
