| 摘要 |
The present invention relates to a method for sequencing a nucleic acid molecule in a touchdown programmed thermocycling reaction which initially comprises a nucleic acid molecule, a first primer, a second primer, a third labelled and internal primer which has a lower annealing temperature than the other primers, four different deoxynucleotides or derivatives thereof, a DNA polymerase, a sequenase, four different dye terminators and one reaction buffer. The present invention also relates to the use of said method and a kit to be used when performing said method. |
