| 摘要 |
The present invention relates generally to the field of nucleic acid detection and diagnostics. More particularly, the present invention relates to a disposable device for detection of specific nucleic acids in a sample. For example, the sample may be a DNA sample amplified using the polymerase chain reaction. The device is operated by aspirating the sample to be analyzed through a tube into a chamber. Inside the chamber, the sample is prepared by contact with pre-measured, pre-deposited reagents. The sample is then processed by mobilization via capillary action through a membrane having pre-measured, pre-deposited signal and/or detection reagents. The sample is analyzed by reading the results which develop on the membrane. For example, specific nucleic acids present in the sample may be detected colorimetrically via hybridization to complementary probes in the membrane. A major advantage of the device is that it provides one-step sample preparation, processing, and analysis. Further, the device is suitable for use outside of a controlled laboratory setting, such as at the point of care and/or in the field. Still further, the device requires no specialized skills to operate. |
