A method for producing a heterologous protein in a lower eukaryotic cell, followed by secretion of said protein from said cell, said protein comprising at least one hydrophobic stretch, wherein the protein is glycosylated such as to increase the level of secretion of the protein, without substantially affecting the functionality of the protein and wherein at least one N-glycosylation group is located between the N-terminus of the protein and the first hydrophobic stretch.
申请公布号
WO0005389(A3)
申请公布日期
2000.06.02
申请号
WO1999EP04919
申请日期
1999.07.08
申请人
UNILEVER N.V.;UNILEVER PLC
发明人
FRENKEN, LEON, GERARDUS, JOSEPH;SAGT, CEES;VERKLEIJ, ARIE, J.;VERRIPS, CORNELIS, THEODORUS