Method for producing protein

摘要

PCT No. PCT/JP95/02186 Sec. 371 Date Sep. 19, 1996 Sec. 102(e) Date Sep. 19, 1996 PCT Filed Oct. 25, 1994 PCT Pub. No. WO96/12816 PCT Pub. Date May 2, 1996A method for producing a desired protein, which comprises growing, by a fed-batch culture, a host cell capable of expressing the desired protein, wherein the specific growth rate of the host cell is changed from the initial rate to a predetermined one by successively changing the rate of addition of a substrate which controls the growth of the host cell. According to the mode of change of the rate of substrate addition to the medium of the present invention, optimal patterns of specific growth rate mu and specific production rate rho can be realized to optimize the fed-batch culture system. As a consequence of the realization, it is made possible to perform a high density culture of the host cell by fed-batch culture, an the desired protein can be produced efficiently in a short time.