发明名称 |
Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity |
摘要 |
This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the cysteine with a substituent group providing a thiol side chain comprising a moiety selected from the group consisting of a polar aromatic substituent, an alkyl amino group with a positive charge, and a glycoside. In particularly preferred embodiments, the substitutents include an oxazolidinone, a C1 to C15 alkyl amino group with a positive charge, or a glycoside. |
申请公布号 |
AU3099000(A) |
申请公布日期 |
2000.05.29 |
申请号 |
AU20000030990 |
申请日期 |
1999.11.09 |
申请人 |
GENENCOR INTERNATIONAL, INC. |
发明人 |
JOHN BRYAN JONES;MICHAEL DICKMAN |
分类号 |
C12N9/48;C12N9/16;C12N9/54;C12N9/78;C12P41/00 |
主分类号 |
C12N9/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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