| 摘要 |
Methods are provided for the rapid, substantially isothermal, amplification of the sequence information of a target nucleic acid positioned within a single- or double-stranded polynucleotide fragment. The methods are based on the serial generation of double-stranded DNA engineered to contain terminal nicking sites, nicking of at least one of those sites, and extensions from the nick(s), thereby displacing any existing polynucleotides. A kit combining the components commonly used in practicing methods of the invention is also provided. |
