Method for labeling nucleotides by reacting a reactive platinum compound having two reactive moieties, a labeled moiety and another moiety of a spacer with a nucleotide

摘要

A method for improved labeling of nucleotides comprised by the steps of: reacting a reactive moiety of a linker of the Formula (I), wherein X represents any stabilising bridge, A and B represents the same or different reacting moieties, with an electron donating moiety (aromatic amine or thiolate anion) of a spacer, which spacer comprises a chain having at least four atoms and at least one heteroatom (oxygen) in the chain, which spacer further comprises said electron donating moiety at one end of the chain and a reactive moiety at the other end of the chain; reacting the reactive moiety of said spacer with a label (biotin, avidin, streptavidin, digoxygenin or a functional equivalent thereof); .reacting the other reactive moiety of said linker with a nucleotide (adenine, thymidine, cytosine, guanine or uridine) A major advantage of this method is that all nucleotides can be labeled by the method described, whereas until now the attachment of a label was mostly restricted to one or certain nucleotides.