| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new C-terminal peptide splitting reagent which contains a lower trialklysilanol alkaline metal salt and a trialkylamine N-oxide by a constitution wherein the N-terminal of a peptide sample is covalent-bonded to an activation PE-COOH solid phase. SOLUTION: A splitting agent is used in methanol, triethylsilylethanol or similar alcohol or in a solvent in a wider range in the case of a triethylamine N-oxide at a molar concentration of about 0.1 to about 0.2. A splitting reaction contains the formation of an unstable C-terminal trimethylsilylester. A C-terminal carboxyl group is reformed quickly under the existence of the ester, water or alcohol. In assition, a splitting agent which is expressed by a formula of R3SiO-X+ (where R represents a hydrocarbon residue and X represents alkali metal ions) is contained. A support contains a nonporous polyethylene film which is modified by an activation carboxylic acid. The thickness range of a PE-COOH film is to be hydrophobic at 0.5 to 2.0 mil.
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