| 摘要 |
A method of separating a soluble milk component from milk is disclosed. The method involves the use of tangential flow filtration across a membrane to form a retentate and a permeate, subjecting the permeate to a capture device to substantially remove the soluble milk component, combining the effluent from the capture device with the original milk sample (retentate), and continuing this procedure until the milk has been sufficiently purified. Preferably, the milk is combined with a chelating agent, such as EDTA, to improve the purification efficiency. This procedure is advantageously employed with milk from transgenic animals that have been genetically altered to express exogenous proteins, such as therapeutic proteins, in their milk.
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