| 摘要 |
<p>PURPOSE: The invention relates to genes capable of conferring resistance against nematodes and/or aphids. Preferred nucleic acids of the invention are DNA sequences which are at least part of the DNA sequence provided on figures or homologous thereto. The invention further relates to vectors, cells and seeds comprising said nucleic acids, as well as genetically transformed plants which are resistant to nematodes and/or aphids. The invention also relates to oligonucleotides, primers, diagnostic kit and polypeptides. CONSTITUTION: A nucleic acid comprising the Mi resistance gene which when present and expressed in a plant is capable of conferring to the plant resistance against nematodes and/or aphids is provided. Furthermore, the Mi resistance gene of which the DNA sequence is provided herein. A gene product encoded by the Mi resistance gene is also provided. In addition, DNA constructs, cosmids, vectors, bacterial strains, yeast cells and plant cells comprising the Mi resistance gene are provided. In another aspect, a genetically transformed plant is provided, which is resistant to a nematode, the nematode being capable of infecting the untransformed plant. Furthermore, the invention relates to resistance genes which are homologous to the Mi resistance gene, and which, when present in a plant, are able of conferring the plant resistance to infection by pathogens. Moreover, the nucleic acid comprises the Meu-1 resistance gene which when present in a plant is capable of conferring to the plant reduced susceptibility to aphids. In particular the Meu-1 resistance gene corresponds to the Mi resistance gene. Especially the Meu-1 resistance gene has the same nucleotide sequence as the Mi resistance gene. Thus, genetically transformed plants, which are reduced susceptible, and preferably resistant to aphids is also provided, in particular to potato aphids. Finally, oligonucleotides corresponding to the sequence of the said resistance gene or part thereof, and detection kits comprising the oligonucleotides are provided.</p> |
