METHOD FOR PRODUCING ANTIBODIES ACTING AGAINST A POLYPEPTIDETHAT ONLY RECOGNISES THE CODING NUCLEIC ACID

摘要

The present invention relates to a method for producing antibodies reacting specifically with a polypeptide that only recognises the coding nucleic acid , wherein said method comprises the following steps: a) the DNA coding the polypeptide is expressed in a host cell using a vector having at least one sequence coding a detection signal, and the expressed polypeptide is bound t o a solid phase using the detection signal; b) regardless of step a), the DNA coding the polypeptide is introduced directly into an animal, which causes t he expression of the polypeptide in the animal thus inducing the formation of antibodies acting against the polypeptide; and c), the polypeptide produced in step a) is used for reacting the antibodies formed in step b), for detecting them or for enriching them.