| 摘要 |
<p>A suspension of cells in a liquid medium is enclosed in a vessel (12), the vessel comprising a plurality of equally-spaced chambers (24) forming a linear array, each chamber communicating with the next chamber in the array via a linking port (26) defined by a smoothly rounded wall portion. The chambers (24) may be of circular cross-section. The liquid medium may be supplied continuously to the vessel by a pump (16), while a piston (18) subjects the liquid medium to an alternating flow which ensures that the contents of each chamber (24) are well mixed and that the residence time for cells in the vessel (12) is substantially uniform. The residence time may be for example 24 or 48 hours. The cells can therefore be cultured continuously.</p> |
