A screening assay for identifying a selective IPC synthase inhibitor which assay comprises contacting a test compound with engineered cells whose capability to synthesize sphingolipids depends on the addition of exogenous phytosphingosine and which are capable of sustained growth via compensatory phospholipids, adding phytosphingosine, and determining IPC synthase inhibition by the test compound by reference to any cell growth inhibition. Engineered cells for use in the assay.
申请公布号
WO0029590(A1)
申请公布日期
2000.05.25
申请号
WO1999GB03789
申请日期
1999.11.12
申请人
ASTRAZENECA UK LIMITED;SCHNELL, NORBERT, FRIEDEMANN;CHAVDA, JINI, SUBERNA