| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for subtracting nucleic acid, capable of simply recovering a cDNA originated from an mRNA specifically expressed in an objective sample in a high recovery rate, and to provide a device therefor. SOLUTION: This method for subtracting nucleic acid comprises using a microplate wherein the surfaces of wells are coated with carbodiimide capable of covalently bonding to nucleic acids such as DNA and RNA, bonding and immobilizing the genetic expression products of a control sample to the wells through the carbodiimide, and subsequently adding a complementary single strand cDNA synthesized using an mRNA of the objective sample as a template, thus hybridizing and immobilizing the cDNA with the complementary mRNA immobilized on the wells. By the reaction, the genes common to both the samples are immobilized on the wells. The supernatant is removed to recover the cDNA originated from the mRNA specific only to the objective sample.
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