发明名称 |
Multiplex selection technique for identifying protein-binding sites and DNA-binding proteins |
摘要 |
Methods for simultaneously selecting binding site sequences for multiple DNA-binding proteins are provided. A source of DNA-binding proteins is mixed with oligonucleotide duplexes containing a randomized internal sequence. Bound oligonucleotides are isolated, amplified and analyzed, such as by DNA sequence analysis. The oligonucleotide duplexes are also used to identify and isolate DNA-binding proteins.
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申请公布号 |
US6066452(A) |
申请公布日期 |
2000.05.23 |
申请号 |
US19970906691 |
申请日期 |
1997.08.06 |
申请人 |
YALE UNIVERSITY |
发明人 |
WEISSMAN, SHERMAN M.;NALLUR, GIRISH N.;KULKARNI, PRAKASH |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C12N15/63 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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