USE OF MAGNESIUM (MG2+) IN PREPARATION OF THERAPEUTIC COMPOSITION FOR TRANSFECTION OF POLYNUCLEOTIDE INTO CELL AND COMPOSITION USEFUL IN GENE THERAPY

摘要

PROBLEM TO BE SOLVED: To dramatically improve the transfection efficiency by adding magnesium to a therapeutic composition for transfecting a polynucleotide into a vertebrate tissue. SOLUTION: Magnesium(Mg2+) is added to a therapeutic composition for in vivo transfection of a polynucleotide into a cell. The magnesium is preferably magnesium chloride. The therapeutic composition preferably contains 0.1-100 mM, preferably 0.1-10 mM of the magnesium. The therapeutic composition is preferably used for administration to a target cell (preferably muscle) of a vertebrate. The composition preferably contains the polynucleotide and the concentration of the polynucleotide in the composition is preferably 0.1μg/ml to 20 mg/ml. Thereby, the composition useful for gene therapy, vaccination, etc., can be obtained.