发明名称 |
OVEREXPRESSION OF DESIRED PROTEINS IN EUKARYOTIC CELLS MEDIATED BY CYCLIN D1 OVEREXPRESSION |
摘要 |
The invention concerns eukaryotic cells useful in protein expression comprising (a) an inserted nucleic acid encoding a cyclin D gene product and (b) an inserted nucleic acid encoding a protein of interest, wherein the cyclin D gene product and the protein of interest are expressed in the cell. The invention also concerns a process for producing a protein of interest, which comprises (a) inserting into a eukaryotic cell a nucleic acid encoding a cyclin D gene product and a nucleic acid encoding a protein of interest; (b) culturing the cell under conditions permitting the expression of the protein of interest; and (c) isolating the protein of interest. The cells are preferably mammalian, with CHO cells most preferred. The cyclin D gene product is preferably of human origin. Suitable proteins of interest include erythropoietin (EPO), osteoprotegerin (OPG), OPG-Fc, leptin, Fc-leptin, and Novel Erythropoiesis Stimulating Protein (NESP).
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申请公布号 |
WO0009714(A9) |
申请公布日期 |
2000.05.18 |
申请号 |
WO1999US18213 |
申请日期 |
1999.08.11 |
申请人 |
AMGEN INC. |
发明人 |
HU, SHAW-FEN, SYLVIA;GUDAS, JEAN, MARIE;BRANKOW, DAVID, WILLIAM |
分类号 |
C12N15/09;C07K14/47;C07K14/475;C12N5/10;C12N15/67;C12P21/02;(IPC1-7):C12N15/67 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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