| 摘要 |
<p>A quantitative assay for an analyte of interest in blood samples, comprising (a) the preparation of a sample for analysis; (b) a kinetic photometric measurement of the sample, being periodic measurements recorded under a controlled temperature, at a selected wavelength in the range of about 330-360 nm; said assay being characterised by (c) a photometric measurement of the sample at a wavelength in the range of 400-420 nm or 535-545 nm or 565-580 nm; (d) conducting steps equivalent to (b) and (c) above upon a control; and (e) calculating the result of the analysis by solving the following δODsμ1/min/δODcμ1/min / ODsμ2/ODcμ2 x Control Value = Sample Value (Activity in U/g Hb) wherein δODs is the change in optical density (per minute) for the sample, μ1 is the wavelength at which optical density is measured under kinetic mode, δODc is the change in optical density (per minute) of a control measured under kinetic mode conditions at a particular wavelength (μ1), ODs is the optical density for the sample measured once at a particular wavelength (μ2), and ODc is the optical density of the control measured once at that particular wavelength (μ2), μ2 is the wavelength at which a reading of Haemoglobin contained can be taken, the control value being determined by the change in optical density (per minute) of the control measured under kinetic mode at μ1 divided by the optical density of the control at μ2 i.e. δODcμ1/min/ODcμ2.</p> |
