发明名称 |
ASSAY FOR PHOSPHATASE-TARGETING TOXINS |
摘要 |
The invention provides an assay method for determining phosphatase targeting toxins which inhibit protein phosphatases comprising contacting a solid support having an immobilized ligand immobilized thereon with: (i) a sample suspected of being contaminated with toxin and (ii) a non-immobilized ligand , wherein said immobilized ligand is capable of generating directly or indirectly detectable signal when uncomplexed, when complexed by said toxin, when complexed by a complex of said toxin and said non-immobilized ligand or when complexed by said non-immobilized ligand or said non-immobilized ligand is capable of generating a directly or indirectly detectable signal when uncomplexed or when complexed, separating a bound fraction from a non-bound fraction; and directly or indirectly determining the non-immobilized ligand bound to the immobilized ligand (the bound fraction) or non-complexed in aqueous solution (the non-bound fraction).
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申请公布号 |
CA2349942(A1) |
申请公布日期 |
2000.05.18 |
申请号 |
CA19992349942 |
申请日期 |
1999.11.11 |
申请人 |
BIOSENSE LABORATORIES AS |
发明人 |
FLADMARK, KARI ESPOLIN;DOSKELAND, STEIN OVE;SERRES, MARGRETHE HAUGE |
分类号 |
C12Q1/42;G01N33/53;G01N33/553;(IPC1-7):C12Q1/42 |
主分类号 |
C12Q1/42 |
代理机构 |
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主权项 |
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地址 |
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