| 摘要 |
<p>A method for introducing mutations into a gene characterized in that a larger number of point mutations are introduced into one strand of a double-stranded genomic DNA of a cell or an organism individual than into the other strand. Use of this method makes it possible to efficiently and effectively construct various useful mutants of microorganisms, cells or organism individuals. By analyzing the mutation conditions of the gene, moreover, it becomes possible to clarify the mechanism of drug tolerance, to estimate the occurrence of a novel insensible bacterium or to develop a drug therefor, to analyze the mutation of an oncogene and the mechanisms of cancer metastasis and increase in malignancy, and to develop methods for using these mechanisms.</p> |
