发明名称 |
RENILLA LUCIFERASE AND GREEN FLUORESCENT PROTEIN FUSION GENES |
摘要 |
A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity. |
申请公布号 |
EP0934425(A4) |
申请公布日期 |
2000.05.17 |
申请号 |
EP19970943558 |
申请日期 |
1997.09.24 |
申请人 |
LOMA LINDA UNIVERSITY |
发明人 |
SZALAY, ALDAR, A.;WANG, GEFU;WANG, YUBAO |
分类号 |
C12N15/09;C07K14/00;C07K14/435;C07K16/00;C07K16/40;C07K19/00;C12N9/02;C12N15/02;C12P21/04;C12P21/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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