HIGH FIDELITY THERMOSTABLE LIGASE AND USES THEREOF
摘要
The present invention is directed to a thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.
申请公布号
WO0026381(A2)
申请公布日期
2000.05.11
申请号
WO1999US25437
申请日期
1999.10.29
申请人
CORNELL RESEARCH FOUNDATION, INC.;BARANY, FRANCIS;CAO, WEIGUO;TONG, JIE