发明名称 |
QUANTIFIZIERUNG VON AN KOHLENHYDRATEN VERARMTEM TRANSFERRIN BEI HOHEM ALKOHOLKONSUM MITTELS HPLC |
摘要 |
A method for determining the amount of carbohydrate deficient transferrin (CDT) in blood serum involves saturating a sample of the blood serum with iron to provide isoforms of transferrin, separating the isoforms of transferrin from other serum proteins in the sample, and developing a chromatogram which relates the separated isoforms with different pI values. The separation step is preferably carried out in an ion exchange column using a salt gradient and the chromatogram is preferably developed using a high performance liquid chromatograph (HPLC). The method is particularly useful for identifying heavy alcohol consumption in a person, which is related to isoforms of transferrin having pI values of 5.9 and 5.7. |
申请公布号 |
DE69423894(D1) |
申请公布日期 |
2000.05.11 |
申请号 |
DE1994623894 |
申请日期 |
1994.07.11 |
申请人 |
BIOLIN MEDICAL AB, STOCKHOLM |
发明人 |
JEPPSSON, OLOF |
分类号 |
C07K14/79;G01N30/02;G01N30/06;G01N30/14;G01N30/26;G01N30/34;G01N30/74;G01N30/88;G01N33/68;G01N33/98;(IPC1-7):G01N33/68 |
主分类号 |
C07K14/79 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|