MULTI-WELL MICROFILTRATION APPARATUS

摘要

The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RN A) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including: (i) a column plate having an array of minicolumns into which samples can be placed; (ii) a discrete filte r element disposed in each minicolumn; (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress; and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to: (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents with in substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter element s disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due toaerosol formation, pendant drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.