发明名称 |
DETECTION OF NEGATIVE-STRAND RNA VIRUSES |
摘要 |
A diagnostic assay for detecting a negative-strand RNA virus in a sample and a genetically engineered cell for use in the assay are disclosed. The cell expresses a heterologous DNA-dependent RNA polymerase that synthesizes a minigenome or miniantigenome of the RNA virus from a cDNA template present in the cell. The cell also expresses the nucleocapsid proteins of the negative-strand virus that are necessary for replication of the minigenome or miniantigenome. Infection of the cell by the negative-strand virus results in expression of a reporter gene product encoded by the miniantigenome.
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申请公布号 |
WO0026417(A1) |
申请公布日期 |
2000.05.11 |
申请号 |
WO1999US25390 |
申请日期 |
1999.10.28 |
申请人 |
WASHINGTON UNIVERSITY |
发明人 |
OLIVO, PAUL, D.;SCHLESINGER, SONDRA;PEEPLES, MARK, E. |
分类号 |
C12N15/09;C12N5/10;C12Q1/68;C12R1/91;(IPC1-7):C12Q1/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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