METHOD FOR MAKING COMPLEMENTARY OLIGONUCLEOTIDE TAG SETS
摘要
The invention provides a method of synthesizing a repertoire of oligonucleotide tags comprising the steps of synthesizing a repertoire of oligonucleotide tag complements on one or more solid phase supports, cleaving a fraction of the oligonucleotide tag complements from the support(s), and inserting the cleaved tag complements into a cloning vector, as depicted in the figure. The cloned tag complements can conventionally be conjugated to selected polynucleotides to produce tagged polynucleotides having unique tag sequences which can be captured and sorted by hybridization to corresponding tag complements. Also provided are various reagents and components that are used or produced in the method.
申请公布号
WO0026411(A1)
申请公布日期
2000.05.11
申请号
WO1999US25680
申请日期
1999.11.01
申请人
LYNX THERAPEUTICS, INC.;WILLIAMS, STEVEN, R.;KIRCHNER, JAMES, J.;DUBRIDGE, ROBERT, B.
发明人
WILLIAMS, STEVEN, R.;KIRCHNER, JAMES, J.;DUBRIDGE, ROBERT, B.