YN87448病毒毒株全基因组序列及其克隆方法

摘要

一种YN87448病毒毒株全基因组序列及其克隆方法,该克隆方法将全基因分15片段,用RT－PCR方法扩增相应cDNA,连入pGEM－T载体,利用通用引物测序,在病毒基因序旬扩增引物设计上,选择甲病毒保守区域的核苷酸序列设计引物,引物3’末端尽可能终止在简并密码子较少的氨基酸位置且最后一个碱基终止在氨基酸密码子的第二位碱基,本发明从分子水平阐明了本病毒与其他流行株的差异,对了解病毒的来源进化过程及分子流行病学有重要意义。也表明:它是预防辛德毕斯脑炎流行的最佳制备疫苗的候选株。

主权项

1、一种YN87448病毒毒株全基因组序列，其特征在于：其全长包括11717个核苷酸(不包括5’端的帽子结构、3’端的多聚核苷酸尾)序列如下：ATTGGCGGCGTAGTACACACTATTGAATTAAACAGCCGACCAATTGCACTACCATCACAATGGAGAAGCCAGTAGTTAACGTAGACGTAGACCCTCAGAGTCCGTTTGTCGTGCAACTGCAAAAGAGCTTCCCGCAATTTGAGGTAGTAGCACAGCAGGTCACTCCAAATGACCATGCTAATGCCAGAGCATTTTCGCATCTGGCCAGTAAACTAATCGAGCTGGAGGTTCCTACCACAGCGACGATTTTGGACATAGGCAGCGCACCGGCTCGTAGAATGTTTTCCGAGCACCAGTACCATTGCGTTTGCCCCATGCGTAGTCCAGAAGACCCGGACCGCATGATGAAATATGCCAGCAAACTGGCGGAAAAAGCATGTAAGATTACAAACAAGAACTTGCATGAGAAGATCAAGGACCTCCGGACCGTACTTGATACACCGGATGCTGAAACGCCATCACTCTGCTTCCACAACGATGTTACCTGCAACACGCGTGCCGAGTACTCCGTCATGCAGGACGTGTACATCAACGCTCCCGGAACTATTTACCACCAGGCTATGAAAGGCGTGCGGACCCTGTACTGGATTGGCTTCGACACCACCCAGTTCATGTTCTCGGCTATGGCAGGTTCGTACCCTGCATACAACACCAACTGGGCCGACGAAAAAGTCCTTGAAGCGCGTAACATCGGACTCTGCAGCACAAAGCTGAGTGAAGGCAGGACAGGAAAGTTGTCGATAATGAGGAAGAAGGAGTTTAAGCCCGGGTCACGGGTTTATTTCTCCGTTGGATCGACACTTTACCCAGAACACAGAGCCAGCTTGCAGAGCTGGCATCTTCCATCGGTGTTCCACTTGAAAGGAAAGCAGTCGTACACTTGCCGCTGTGATACAGTGGTGAGCTGCGAAGGCTACGTAGTGAAGAAAATCACCATCAGTCCCGGGGTCACGGGAGAAACCGTGGGATACGCGGTTACATACAATAGCGAGGGCTTCTTGCTATGCAAAGTTACCGATACAGTAAAAGGAGAACGGGTATCGTTCCCCGTGTGCACGTATATCCCGGCCACCATATGCGATCAGATGACCGGCATAATGGCCACGGATATCTCACCTGACGATGCACAAAAACTTCTGGTTGGGCTCAACCAGCGAATCGTCATTAACGGTAAGACTAACAGGAACACCAATACCATGCAAAATTACCTTCTGCCAATCATTGCACAAGGGTTCAGCAAATGGGCCAAGGAGCGCAAAGCAGATCTTGACAATGAAAAAATGCTGGGCACCAGAGAGCGCAAGCTTACATATGGCTGCTTGTGGGCGTTTCGCACTAAGAAAGTGCACTCGTTCTATCGCCCACCTGGAACGCAGACCAGCGTAAAAGTCCCAGCCTCTTTTAGCGCTTTCCCCATGTCATCCGTATGGACTACCTCTTTGCCCATGTCGCTGAGGCAGAAGGTGAAATTGGCATTACAACCAAAGAAGGAGGAAAAACTGCTGCAAGTCCCGGAGGAATTAGTTATGGAGGCCAAGGCTGCTTTCGAGGATGCTCAGGAGGAATCCAGAGCGGAGAAGCTCCGAGAAGCACTCCCACCATTAGTGGCAGACAAAGGTATCGAGGCAGCTGCGGAAGTTGTCTGCGAAGTGGAGGGGCTCCAGGCGGACATCGGAGCAGCACTCGTCGAAACCCCGCGCGGTCATGTAAGGATAATACCTCAAGCAAATGACCGTATGATCGGACAGTATATCGTTGTCTCGCCGATCTCTGTGCTGAAGAACGCTAAACTCGCACCAGCACACCCGCTGGCAGACCAGGTTAAGATCATAACGCACTCCGGAAGATCAGGAAGGTATGCAGTCGAACCATACGACGCTAAAGTACTGATGCCAGCAGGAAGTGCTGTACCATGGCCAGAATTCTTAGCACTGAGTGAGAGCGCCACGCTAGTGTACAACGAAAGAGAGTTTGTGAACCGCAAGCTGTACCATATTGCCATGCACGGTCCCGCTAAGAATACAGAAGAGGAGCAGTACAAGGTTACAAAGGCAGAGCTCGCAGAAACAGAGTACGTGTTTGACGTGGACAAGAAGCGATGCGTTAAGAAGGAAGAAGCCTCAGGACTTGTCCTTTCGGGAGAACTGACCAACCCGCCCTATCACGAACTAGCTCTTGAGGGACTGAAGACTCGACCCGCGGTCCCGTACAAGGTTGAAACAATAGGAGTGATAGGCACACCAGGATCGGGCAAGTCAGCTATCATCAAGTCAACTGTCACGGCACGTGATCTTGTTACCAGCGGAAAGAAAGAAAACTGCCGCGAAATTGAGGCCGACGTGCTACGGCTGAGGGGCATGCAGATCACGGCGAAGACAGTGGATTCGGTTATGCTCAACGGATGCCACAAAGCCGTAGAAGTGCTGTATGTTGACGAAGCGTTCGCGTGCCACGCAGGAGCACTACTTGCCTTGATTGCAATCGTCAGACCCCGTAAGAAGGTAGTACTATGCGGAGACCCTAAGCAATGCGGATTCTTCAACATGATGCAACTAAAGGTACATTTCAACCACCCTGAAAAAGACATATGTACCAAGACATTCTACAAGTTTATCTCCCGACGTTGCACACAGCCAGTCACGGCTATTGTATCGACACTGCATTACGATGGAAAAATGAAAACCACAAACCCGTGCAAGAAGAACATCGAAATCGACATTACAGGGGCCACGAAGCCGAAGCCAGGGGACATCATCCTGACATGTTTCCGCGGGTGGGTTAAGCAACTGCAAATCGACTATCCCGGACATGAGGTAATGACAGCCGCGGCCTCACAAGGGCTAACCAGAAAAGGAGTATATGCCGTCCGGCAAAAAGTCAATGAAAACCCGCTGTACGCGATCACATCAGAGCATGTGAACGTGTTGCTCACCCGCACTGAGGACAGGCTAGTATGGAAAACTTTACAGGGCGACCCATGGATTAAGCAGCTCACTAACGTACCTAAAGGAAATTTTCAGGCCACCATCGAGGACTGGGAAGCTGAACACAAGGGAATAATTGCTGCGATAAACAGTCCCGCTCCCCGTACCAATCCGTTCAGCTGCAAGACTAACGTTTGCTGGGCGAAAGCACTGGAACCGATACTGGCCACGGCCGGTATCGTACTTACCGGTTGCCAGTGGAGCGAGCTGTTCCCACAGTTTGCGGATGATAAACCACACTCGGCCATCTACGCCTTAGACGTAATTTGCATTAAGTTTTTCGGCATGGACTCGACAAGCGGACTGTTTTCCAAACAGAGCATCCCGTTAACGTACCATCCTGCCGACTCAGCGAGGCCAGTAGCTCATTGGGACAACAGCCCAGGAACACGCAAGTATGGGTACGATCACGCCGTTGCCGCCGAACTCTCCCGTAGATTTCCGGTGTTCCAGCTAGCTGGGAAAGGCACACAGCTTGATTTGCAGACGGGCAGAACTAGAGTTATCTCTGCACAGCATAACTTGGTCCCAGTGAACCGCAATCTCCCTCACGCCTTAGTCCCCGAGCACAAGGAGAAACAACCCGGCCCGGTCGAAAAATTCTTGAGCCAGTTCAAACACCACTCCGTACTTGTGATCTCAGAGGAAAAAATTGAAGCTCCCCACAAGAGAATCGAATGGATCGCCCCGATTGGCATAGCCGGCGCAGATAAGAACTACAACCTGGCTTTCGGGTTTCCGCCGCAGGCACGGTACGACCTGGTGTTCATCAATATTGGAACTAAATACAGAAACCATCACTTTCAACAGTGCGAAGACCACGCGGCGACCTTGAAAACCCTTTCGCGTTCGGCCCTGAACTGCCTTAACCCCGGAGGCACCCTCGTGGTGAAGTCCTACGGTTACGCCGACCGCAATAGTGAGGACGTAGTCACCGCTCTTGCCAGAAAATTTGTCAGAGTGTCTGCAGCGAGGCCAGAGTGCGTCTCAAGCAATACAGAAATGTACCTGATTTTCCGACAACTAGACAACAGCCGCACACGACAATTCACCCCGCATCATTTGAATTGTGTGATTTCGTCCGTGTACGAGGGTACAAGAGACGGAGTTGGAGCCGCACCGTCGTACCGTACTAAAAGGGAGAACATTGCTGATTGTCAAGAGGAAGCAGTTGTCAATGCAGCCAATCCACTGGGCAGACCAGGAGAAGGAGTCTGCCGTGCCATCTATAAACGTGGGCCGAACAGTTTCACCGATTCAGCCACAGAGACAGGCACCGCAAAACTGACTGTGTGCCAAGGAAAGAAAGTGATCCACGTGGTTGGCCCTGATTTCCGGAAACACCCAGAGGCAGAAGCCCTGAAATTGCTGCAAAACGCCTACCATGCAGTGGCAGACTTAGTAAATGAACATAATATCAAGTCTGTCGCCATCCCATTGCTATCTACAGGCATTTACGCAGCCGGAAAAGACCGCCTTGAGGTATCACTTAACTGCTTGACAACCGCGCTAGACAGAACTGATGCGGACGTAACCATCTACTGCCTGGATAAGAAGTGGAAGGAAAGAATCGACGCGGTGCTCCAACTTAAGGAGTCTGTAACTGTGCTGAAGGATGAGGATATGGAGATCGACGACGAGTTAGTATGGATCCATCCGGACAGTTGCCTGAAGGGAAGACAGGGATTCAGTACTACAAAAGGAAAGTTGTATTCGTACTTTGAAGGCACCAAATTCCATCAAGCAGCAAAAGATATGGCGGAGATAAAGGTCCTGTTCCCAAATGACCAGGAAAGCAACGAACAACTGTGTGCCTACATATTGGGGGAGACCATGGAAGCAATCCGCGAAAAATGCCCGGTCGACCACAACCCGTCGTCTAGCCCGCCAAAAACGCTGCCGTGCCTCTGTATGTATGCCATGACGCCAGAAAGGGTCCACAGACTCAGAAGCAATAACGTTAAAGAAGTTACAGTATGCTCCTCCACCCCCCTTCCAAAGTACAAAATCAAGAATGTTCAGAAGGTTCAGTGCACAAAAGTAGTCCTGTTTAACCCGCATACCCCCGCATTCGTTCCCGCCCGTAAGTACATAGAAGCACCAGAACAGCCTGCAGCTCCGCCTGCACAGGCCGAGGAGGCCCCCGGAGTTGTAGCGACACCAACACCACCTGCAGCTGATAACACCTCGCTTGATGTCACGGACATCTCACTGGACATGGAAGACAGTAGCGAAGGCTCACTCTTTTCGAGCTTTAGCGGATCGGACAACTCTATTACTAGTATGGACAGTTGGTCGTCAGGACCTAGTTCACTAGAGATAGTAGACCGAAGGCAGGTGGTGGTGGCTGACGTCCATGCCGTCCAAGAGCCTGCCCCTGTTCCACCGCCAAGGCTAAAGAAGATGGCCCGCCTGGCAGCGGCAAGAATGCAGGAAGGGCCAACTCCACCGGCAAGCACCAGCTCTGCGGACGAGTCCCTTCACCTTTCTTTTGATGGGGTATCTATGTCCTTCGGATCCCTTTTCGACGGAGAGATGGTCCGCTTGGCAGCGGCACAACCCCCGGCAACATGCCCTACGGATGTGCCTATGTCTTTCGGATCGTTTTCCGACGGAGAGATTGAGGAGTTGATCCGCAGAGTAACCGAGTCGGAGCCCGTCCTGTTTGGGTCATTTGAACCGGGCGAAGTGAACTCAATTATATCGTCCCGTTCAGCCGTATCTTTTCCACCACGCAAGCAGAGACGTAGACGCAGGAGCAGGAGGACCGAATACTGACTAACCGGGGTAGGTGGGTACATATTTTCGACGGACACAGGCCCTGGGCACTTGCAAAAGAAGTCCGTTCTGCAGAACCAGCTTACAGAACCGACCTTGGAGCGCAATGTTCTGGAAAGAATCTACGCCCCGGTGCTCGACACGTCGAAAGAGGAACAGCTCAAACTCAGGTACCAGATGATGCCCACCGAAGCCAACAAAAGCAGGTACCAGTCTCGAAAAGTAGAAAACCAGAAAGCCATAACCACTGAGCGACTGCTTTCAGGGCTACGACTGTATAACTCTGCCACAGATCAGCCAGAATGCTATAAGATCACCTACCCGAAACCATCGTATTCCAGCAGTGTACCAGCTAACTACTCTGACCCAAAGTTTGCTGTAGCTGTTTGTAACAACTATCTGCATGAGAATTACCCGACGGTAGCATCTTATCAGATCACCGACGAGTACGATGCTTACTTGGATATGGTAGACGGGACAGTCGCTTGCCTAGATACTGCAATTTTTTGCCCCGCCAAGCTTAGAAGTTACCCGAAAAGACACGAGTATAGAGCCCCAAACATCCGCAGTGCGGTTCCATCAGCGATGCAGAACACGTTGCAAAACGTGCTCATTGCCGCGACTAAAAGAAACTGCAACGTCACACAAATGCGTGAACTGCCAACACTGGACTCAGCGACATTCAACGTTGAATGCTTTCGAAAATATGCATGCAATGACGAGTATTGGGAGGAGTTTGCCCGAAAGCCAATTAGGATCACTACTGAGTTCGTTACCGCATACGTGGCCAGACTGAAAGGCCCTAAGGCCGCCGCACTGTTCGCAAAGACGCATAATTTGGTCCCATTGCAAGAAGTGCCTATGGATAGATTCGTCATGGACATGAAAAGAGACGTGAAAGTTACACCTGGCACGAAACACACAGAAGAAAGACCGAAAGTACAAGTGATACAAGCCGCAGAACCCCTGGCGACCGCTTACCTATGCGGGATCCACCGGGAGTTAGTGCGCAGGCTTACAGCCGTTTTGCTACCCAACATTCACACGCTCTTTGACATGTCGGCGGAGGACTTTGATGCAATCATAGCAGAACACTTCAAGCAAGGTGACCCGGTACTGGAGACGGATATCGCCTCGTTCGACAAAAGCCAAGACGACGCTATGGCGTTAACCGGCCTGATGATCTTGGAAGACCTGGGTGTGGACCAACCACTACTCGACTTGATCGAGTGCGCCTTTGGAGAAATATCATCCACCCATCTGCCCACGGGTACCCGTTTCAAATTCGGGGCGATGATGAAATCCGGAATGTTCCTCACGCTCTTTGTCAACACAGTTCTGAATGTCGTTATCGCCAGCAGAGTATTGGAGGAGCGGCTTAAAACGTCCAAATGTGCAGCATTTATCGGCGACGATAACATTATACACGGAGTAGTATCTGACAAAGAAATGGCTGAGAGGTGTGCCACCTGGCTCAACCTGGAGGTTAAGATCATTGACGCAGTCATCGGCGAGAGACCACCTTACTTCTGCGGTGGATTCATCTTGCAAGATTCGGTTACCTCCACAGCGTGTCGCGTGGCGGACCCCTTGAAAAGGCTGTTTAAGTTGGGTAAACCGCTCCCAGCCGACGACGAGCAAGACGAAGACAGAAGACGCGCTCTGCTAGATGAAACAAAGGCGTGGTTTAGAGTAGGTATAACAGACACCTTAGCAGTGGCCGTGGCAACTCGGTATGAGGTAGACAACATCACACCTGTCCTGCTGGCATTGAGAACTTTTGCCCAGAGCAAAAGAGCATTTCAAGCCATCAGAGGGGAAATAAGGCATCTCTACGGTGGTCCTAAATAGTCAGCATAGTACATTTCATCTGACTAATACCACAACACCACCACCATGAATAGAGGATTCTTTAACATGCTCGGCCGCCGCCCCTTCCCAGCCCCCACTGCCATGTGGAGGCCGCGGAGAAGGAGGCAGGCGGCCCCGATGCCTGCCCGCAATGGGCTGGCTTCCCAAATCCAGCAACTGACCACAGCCGTCAGTGCCCTAGTCATTGGACAGGCAACTAGACCTCAAACCCCACGCCCACGCCCGCCGCCGCGCCAGAAGAAGCAGGCGCCAAAGCAACCACCGAAGCGGAAGAAACCAAAAACACAGGAGAAGAAGAAGAAGCAACCTGCAAAACCCAAACCCGGAAAGAGACAGCGTATGGCACTTAAGTTGGAGGCCGACAGACTGTTCGACGTAAAAAATGAGGACGGAGATGTCATCGGGCACGCACTGGCCATGGAAGGAAAGGTAATGAAACCTCTCCACGTGAAAGGAACTATTGACCACCCTGTGCTATCAAAGCTCAAATTCACCAAGTCGTCAGCATACGACATGGAGTTCGCACAGTTGCCGGTCAACATGAGAAGTGAGGCGTTCACCTACACCAGTGAACACCCTGAAGGGTTCTACAACTGGCACCACGGAGCGGTGCAGTATAGTGGAGGCAGATTTACCATCCCCCGCGGAGTAGGAGGCAGAGGAGACAGTGGTCGTCCGATTATGGATAACTCAGGCCGGGTTGTCGCGATAGTCCTCGGAGGGGCTGATGAGGGAACAAGAACCGCCCTTTCGGTCGTCACCTGGAATAGCAAAGGGAAGACAATCAAGACAACCCCGGAAGGGACAGAAGAGTGGTCTGCTGCACCACTGGTCACGGCCATGTGCTTGCCTGGAAACGTGAGCTTCCCATGCAATCGCCCGCCCACATGCTACACCCGCGAACCATCCAGAGCTCTCGACATCCTCGAAGAGAACGTGAACCACGAGGCCTACGACACCCTGCTCAACGCCATATTGCGGTGCGGATCGTCCGGCAGAAGTAAAAAAAAAAGCGTCACTGACGACTTTACCTTGACCAGCCCGTACTTGGGCACATGCTCGTACTGTCACCATACTGAACCGTGCTTTAGCCTGATTAAGATCGAGCAGGTCTGGGATGAAGCGGACGACAACACCATACGCATACAGACTTCCGCCCAGTTTGGATACGACCAAAGCGGAGCAGCAAGCTCAAATAAGTACCGCTACATGACGCTCGAGCAGGATCATACTGTCAAAGAAGGCACCATGGATGACATCAAGATCAGCACCTCAGGACCGTGTGGAAGGCTTAGCTACAAAGGATACTTTCTCCTCGCGAAGTGTCCTCCAGGGGACAGCGTAACGGTTAGCATAGCGAGTAGCAACTCAGCAACGTCATGCACAATGGCCCGCAAGATAAAACCAAAATTCGTGGGACGGGAAAAATATGACCTACCTCCCGTTCACGGTAAGAAGATTCCTTGCACAGTGTACGACCGTCTGAAAGAAACAACCGCCGGCTACATCACTATGCACAGGCCGGGACCGCACGCCTATACATCCTATCTGAAGGAATCATCAGGGAAAGTTTACGCGAAGCCACCATCCGGGAAGAACATTACGTACGAGTGCAAGTGCGGCGATTACAAGACCGGAACCGTTACGACCCGTACCGAAATCACGGGCTGCACCGCCATCAAGCAGTGCGTCGCCTATAAGAGCGACCAAACGAAGTGGGTCTTCAACTCGCCGGACTCTATCAGACACGCCGACCACACGGCCCAAGGGAAATTGCATTTGCCTTTCAAGCTGATCCCGAGTACCTGCATGGTCCCTGTTGCCCACGCGCCGAACGTAGTACACGGCTTTAAACACATCAGCCTCCAATTAGACACAGACCATCTGACATTGCTCACCACCAGGAGACTAGGGGCAAACCCGGAACCAACCACTGAATGGATCATCGGAAAGACGGTTAGAAACTTCACCGTCGACCGAGATGGCCTGGAATACATATGGGGCAATCACGAACCAGTAAGGGTCTATGCCCAAGAGTCTGCACCAGGAGACCCTCACGGATGGCCACACGAAATAGTACAGCATTACTATCATCGCCATCCTGTGTACACCATCTTAGCCGTCGCATCAGCTGCTGTGGCGATGATGATTGGCGTAACTGTTGCAGCATTATGTGCCTGTAAAGCGCGCCGTGAGTGCCTGACGCCATATGCCCTGGCCCCAAATGCCGTGATTCCAACTTCGCTGGCACTTTTGTGCTGTGTTAGGTCGGCTAATGCTGAAACATTCACCGAGACCATGAGTTACTTATGGTCGAACAGCCAGCCGTTCTTCTGGGTCCAGCTGTGTATACCTCTGGCCGCTGTCATCGTTCTAATGCGCTGTTGCTCATGCTGCCTGCCTTTTTTAGTGGTTGCCGGCGCCTACCTGGCGAAGGTAGACGCCTACGAACATGCGACCACTGTTCCAAATGTGCCACAGATACCATATAAGGCACTTGTTGAAAGGGCAGGGTACGCCCCGCTCAGTTTGGAGATTACTGTCATGTCCTCGTAGGTTTTGCCTTCCACCAACCAAGAGTACATCACCTGCAAATTCACCACTGTGGTCCCCTCCCCTAAAGTCAGATGCTGCGGCTCCTTGGAATGTCAGCCCGCGGCTCACGCAGATTATACCTGCAAGGTCTTTGGAGGGGTGTACCCCTTCATGTGGGGAGGAGCACAATGTTTTTGCGACAGTGAGAACAGCCAGATGAGTGAGGCGTACGTCGAATTGTCAGCAGATTGCGCGACTGACCACGCGCAGGCGATTAAGGTGCATACTGCCGCGATGAAAGTAGGACTGCGTATAGTGTACGGGAACACTATCAGTTTCCTAGATGTGTACGTGAACGGAGTCACACCAGGAACGTCTAAAGACCTGAAAGTCATAGCTGGACCAATTTCAGCATTGTTTACACCATTCGATCACAAGGTCGTTATCCATCGCGGCCTGGTGTACAACTATGACTTTCCGGAATACGGAGCGATGAAACCAGGAGCGTTTGGAGACATTCAAGCTACCTCCTTGACTAGCAAAGACCTCATCGCCAGCACAGATATTAGGCTACTCAAGCCTTCCGCCAAGAACGTGCATGTCCCGTACACGCAGGCCGCATCTGGATTCGAGATGTGGAAAAACAACTCAGGCCGCCCACTGCAGGAAACCGCCCCTTTTGGGCGCAAGATTGCAGTCAATCCGCTTCAGGCGGTGGACTGCTCATACGGGAACATTCCCATCTCTATTGACATCCCGAACGCTGCCTTTATCAGGACATCAGATGCACCACTGGTCTCAACAGTCAAATGTGATGTCAGTGAGTGCACTTATTCAGCGGACTTCGGCGGGATGGCTACCCTGCAGTATGTATCCGACCGCGAAGGACAATGCCCTGTACATTCGCATTCGAGCACAGCAACCCTCCAAGAGTCGACAGTTCATGTCCTGGAGAAAGGAGCGGTGACAGTACACTTCAGCACCGCGAGCCCACAGGCAAACTTCATTGTATCGCTGTGTGGTAAGAAGACAACATGCAATGCAGAATGCAAACCACCAGCTGATCATATCGTGAGCACCCCGCACAAAAATGACCAAGAATTCCAAGCCGCCATCTCAAAAACTTCATGGAGTTGGCTGTTTGCCCTTTTCGGCGGCGCCTCGTCGCTATTAATTATAGGACTTATGATTTTTGCTTGCAGCATGATGCTGACTAGCACACGAAGATGACCGCTACGCCCCAATGACCCGACCAGCAAAACTCGATGTACTTCCGAGGAACTGATGTGCATAATGCATCAGGCTGGTATATTAGATCCCCGCTTACCGCGGGCAATATAGCAACACCAAAACTCGACGTATTTCCGAGGAAGCGCAGTGCATAATGCTGCGCAGTGTTGCCAAATAATCACTATATTAACCATTTATTCAGCGGACGCCAAAACTCAATGTATTTTTGAGGAAGCATGGTGCATAATGCCATGCAGCGTCTGCATAACTTTTTATTATTTCTTTTATTAATCAACAAAATTTTGTTTTTAACATTTC