发明名称 |
Method of transducing mammalian cells, and products related thereto |
摘要 |
In accordance with the present invention, there are provided methods of transducing cells comprising providing a flexible closed culture container having cells therein and contacting said cells with a viral-vector in the presence of a multi-functional chemical moiety. Also provided are methods of delivering a functional protein to a subject in need thereof, comprising transducing mammalian cells according to the invention method and introducing said cells into a subject in need thereof. Also provided are cell-culture systems for transducing cells, comprising a flexible closed culture container and a multi-functional chemical moiety therein.
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申请公布号 |
US6060317(A) |
申请公布日期 |
2000.05.09 |
申请号 |
US19980133033 |
申请日期 |
1998.08.11 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF HEALTH AND HUMAN SERVICES |
发明人 |
MALECH, HARRY L. |
分类号 |
C07K14/78;C12M1/24;C12N15/64;C12N15/87;(IPC1-7):C12N15/63 |
主分类号 |
C07K14/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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