发明名称 |
Extracellular expression of cellulose binding domains (CBD) using Bacillus |
摘要 |
The present invention relates to Bacillus hosts transformed with a vector comprising a DNA sequence encoding for a cellulose binding domain (CBD) and capable of expressing said sequence, the expressed polypeptide protein consisting essentially of one or more non-catalytic domains; the cellulose binding domain having a molecular weight in the range of from 4 kD to 35 kD and being obtainable from a microorganism or from a plant, preferably from a bacterium or a fungus; the Bacillus host e.g. being one of the species Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilos, and Bacillus amyloliquefaciens; and a Bacillus expression vector carrying an inserted DNA sequence encoding for a cellulose binding domain; and a method for producing a cellulose binding domain polypeptide in a Bacillus host cell.
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申请公布号 |
US6060274(A) |
申请公布日期 |
2000.05.09 |
申请号 |
US19970959212 |
申请日期 |
1997.10.28 |
申请人 |
NOVO NORDISK A/S |
发明人 |
BJORNVAD, MADS ESKELUND;SCHULEIN, MARTIN;JORGENSEN, PER LINA |
分类号 |
C12N15/09;C07K14/195;C07K14/21;C07K14/33;C12N1/21;C12P21/02;C12Q1/68;C12R1/07;(IPC1-7):C12P21/06;C12N1/20;C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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