| 摘要 |
<p>A device for determining the concentration of a substance mixed with a fluorophor and contained in one or more contact points on a reflecting support having low absorption comprises a microscope (1) with means of taking an image (4, 5), lighting means (11, 13), deflection means (15), recording means (6, 7) and processing means (6, 7). A device for determining the concentration of a substance mixed with a fluorophor and contained in one or more contact points on a reflecting support having low absorption comprises: (1) a microscope (1) combined with an magnifying lens (2) and means of taking an image (4, 5) to produce an image of the florescence of one of the contact points or part of it; (2) lighting means (11, 13) emitting a first beam to allow to take the image in white light of the contact point and a second beam to excite the fluorophor in the contact point; (3) deflection means (15) of the second beam to screen point by point the contact point; (4) recording means (6, 7) of the image of the plot; and (5) processing means (6, 7) of this image to quantify the fluorescence and determine the concentration of the substance mixed with the fluorophor from the intensity of the fluorescence, the intensity of the light and the integration time with respect to the means of taking the image. An Independent claim is also included for a method of determining the concentration of a substance mixed with a fluorophor using the above described device comprising: (1) calibrating the device by positioning and blocking the matrix of the contact points under the optic axis of the objective of the microscope, adjusting the means of taking the image and automatically searching a reference contact point with respect to the optical center of the device; (2) an acquisition step of the image of one contact point; and (3) a calculation step of the fluorescence of the contact point and determination of the concentration of the substance.</p> |
