| 摘要 |
The present invention relates to a method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of: a) providing a TPS gene; b) designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5' terminus of the yeast gene; c) deleting a part of the N-terminal region of the TPS gene extending beyond the 5' terminus of the yeast gene, preferably the complete extending part thereof, in order to achieve an increased trehalose-6-phosphate synthase activity; d) cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; e) transforming a plant cell or tissue with the thus obtained expression vector; and f) regenerating a complete plant from the transformed plant cell or tissue, wherein the plant is selected from Selaginella lepidophylla, Arabidopsis thaliana, rice, apple, sugar beet, sunflower (Helianthus annuus), tobacco (Nicotiana tabacum), soybean (Glycine max); and wherein the fungus is selected from Aspergillus niger, Agaricus bisporus, Pictia pastoris, Kluyveromyces lactis and methylotrophic yeasts, and wherein the animal is selected from mammalian and invertebrate cell cultures used for production of proteins and small molecules, invertebrate cells used for baculovirus expression. Furthermore, the present invention relates to plants having an increased stress tolerance and/or increased photosynthetic productivity and/or improved carbon partitioning in the whole plant or in specific plant parts and harboring in their genome said specifically modified TPS gene. <IMAGE> |
