EXPRESSION VECTOR FOR MAMMAL CELL CONTAINING SHORT INTERPERSED ELEMENTS SEQUENCE (SINE)

摘要

PURPOSE: Provided is an expression vector for a mammal cell containing a short interpersed elements sequence (SINE) wherein SINE inserts an exogenous gene into chromosome, to enhance transformation rate. And a method for transformation using the same is also provided. CONSTITUTION: An expression vector used in a mammal cell for increasing transformation rate has more than one SINE at either 5'end or 3'end, or at both 5'end and 3'end of an exogenous gene. SINE can be B1 sequence, B2 sequence, fusion sequence thereof, ID sequence, Alu sequence or C-BCS sequence, and preferably fusion sequence of B1 and B2 sequence produced by typical ligation reaction. The vectors prepared by using PGKβ -geobp(A) as exogenous gene are as follows: (a) p(B1)2β-geo wherein 2B1 sequences locate at 5'end and 3'end of exogenous gene, respectively; (b) p(B2)2β-geo wherein 2B2 sequences locate at 5'end and 3'end of exogenous gene, respectively; (c) p(B1/2)β -geo wherein fusion sequence of B1 and B2 locates at 3'end of exogenous gene; (d) p(B1/2)2β-geo wherein fusion sequence of B1 and B2 sequences locates at 5'end of exogenous gene.