发明名称 |
FUSION PROTEIN PRES2HV OF SURFACE ANTIGEN PRES2 SAG OF HEPATITIS B VIRUS AND HV-H5 PROTEIN OF HEPATITIS C VIRUS, AND PRODUCING METHOD THEREOF |
摘要 |
PURPOSE: A fusion protein preS2HV of the surface antigen preS2 SAg of hepatitis B virus and HV-H5 protein of hepatitis C virus and a producing method thereof are provided, thereby vaccines having improved specificity and immunity for HBV and HCV can be produced. CONSTITUTION: The fusion protein preS2HV represented by the amino acid sequence of Fig. 1 is produced by the steps of: preparing a primer for pre S2 SAg gene of HBV; amplifying the pre S2 SAg gene by PCR(polymerase chain reaction) using plasmid pYPSAG100 as a template; coupling the amplified pre S2 SAg gene with the ubiquitin gene and amplifying the coupled gene by PCR; cleaving the coupled gene with endonucleases and inserting the fragments into an appropriate expression vector; preparing a primer for HV-H5 gene of HCV; amplifying the HV-H5 gene by PCR using plasmid M13mp18-LBC-HV(ATCC 75448) as a template; and inserting the amplified HV-H5 gene into the expression vector containing the coupled gene of pre S2 SAg and ubiquitin.
|
申请公布号 |
KR100254830(B1) |
申请公布日期 |
2000.05.01 |
申请号 |
KR19940040024 |
申请日期 |
1994.12.30 |
申请人 |
LG CHEMICAL CO.,LTD |
发明人 |
KIM, CHUN HUNG;CHO, JUNG MYEONG |
分类号 |
C12N15/51;C07K14/02;C07K14/18;(IPC1-7):C12N15/51 |
主分类号 |
C12N15/51 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|