发明名称 |
PROTEIN ENGINEERING |
摘要 |
A method of protein engineering is provided wherein a searchable computer database is created comprising entries in the form of descriptions of a location and orientation in 3D space of side chains of the constituent amino acid residues of a framework protein. A query is created which corresponds t o a description of a location and orientation in 3D space of respective side chains of amino acid residues of a sample protein. The location and orientation in 3D space of constituent side-chains is preferably described a s a C.alpha.-C.beta. vector. The query is used to search the database and thereby identify a hit which corresponds to a framework protein having structural similarity with said sample protein. Framework protein "hits" so identified may be suitable candidates for further modification. A particular advantage of the present invention is that a modified framework protein may display one or more desired characteristics, such as a function similar to o r inhibitory of the sample protein.
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申请公布号 |
CA2347917(A1) |
申请公布日期 |
2000.04.27 |
申请号 |
CA19992347917 |
申请日期 |
1999.10.21 |
申请人 |
THE UNIVERSITY OF QUEENSLAND |
发明人 |
DOOLEY, MICHAEL JOHN;ANDREWS, PETER RONALD;SMYTHE, MARK LESLIE |
分类号 |
G01N33/50;C07K1/00;C07K14/535;C07K14/54;C07K14/61;G01N33/15;G01N33/68;G06F17/30;G06F19/00;(IPC1-7):C07K14/61 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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