METHODS FOR DETECTING AND ISOLATING NUCLEAR TRANSPORT PROTEINS

摘要

Whether or not a test DNA encodes a peptide with nuclear transportability can be readily detected by introducing a fusion DNA formed by a DNA encoding a transcription factor whose nuclear transportability is eliminated and the test DNA into a eukaryotic host having in its nucleus a promoter region that is activated when said transcription factor binds thereto and a reporter gene whose expression is induced by said promoter region, and detecting the expression of said reporter gene. A DNA encoding a peptide with nuclear transportability is efficiently and systematically isolated by isolating a test DNA from cells expressing the reporter gene.