发明名称 |
Cdna, genomic, and predicted protein sequences of learning-induced kinases |
摘要 |
To elucidate molecular mechanisms in learning and memory, the expression of mRNAs in brains of rabbitsundergoing eyeblink conditioning was analyzed. Infusion of the transcription inhibitor actinomycin D (ActD) into the cerebellar nuclei reversibly blocked learning but not performance of the CR. Differential display PCR (DD-PCR) analysis of cerebellar RNAs from trained and pseudo-trained rabbits identified a 207-bp band that was induced with learning. The fragment was used to isolate a cDNA from a lambdagt11 rabbit brain library containing a 1698-bp open-reading frame. The genomic sequence also has been obtained and is reported. The deduced amino acid sequence contains the KKIAMRE motif, which is conserved among cdc2-related kinases. These results suggest that there is a new category of cdc2-related kinases in the brain whose function may be important in learning and memory. |
申请公布号 |
AU6285699(A) |
申请公布日期 |
2000.04.26 |
申请号 |
AU19990062856 |
申请日期 |
1999.10.01 |
申请人 |
UNIVERSITY OF SOUTHERN CALIFORNIA |
发明人 |
RICHARD F. THOMPSON;HIROSHI GOMI;WILLIAM SUN |
分类号 |
C12N9/12 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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