| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for individually and quantitatively analyzing cholesterol in each lipoprotein (e.g. respectively in high-density lipoprotein, in low-density one and in very low-density one) without performing a fractionation procedure, which is important for lipid metabolism in the field of clinical diagnosis. SOLUTION: The method of this invention is to directly and selectively measure cholesterol quantity in each lipoprotein (i.e., chylomicron, HDL, LDL, or VLDL) in a specimen in the presence of a phosphorus compound, a surfactant and a solubilizer for dissolving protein. It is possible to impart selectivity to the reaction of each lipoprotein and enzyme by selecting the kind of the phosphorous compound and by selecting the kinds and concentrations of the surfactant and the solubilizer, and to selectively measure cholesterol in each lipoprotein even in a state that various lipoproteins coexist in a specimen.
|
