| 摘要 |
PURPOSE: Colchicinoid compounds are transformed into the corresponding 3-O-glycosyl derivatives by means of Bacillus moratorium strains CONSTITUTION: Provided is a method of bio transformation of colchicinoid compounds, into the respective 3-O-glycosyl derivatives by using selected microbial strains. An operative sequences for the selection of the bacterial strains usable in the process of the invention comprise: A) Selection of cultures of Bacillus meaaterium capable of growing in the presence of high concentrations of colchicinic substrate, starting from natural sources or from collection strains; B) Selection of the isolate from A), to verify the transformation activity of colchicines into the corresponding 3-O-glycosyl derivatives, by means of bio conversion assays on the specific substrates, administered in gradually increasing concentrations; C) Microbiological characterization of the strains selected in B); D) Gradual increase in the bio transformation yield, by means of a target-specific selection of the bacterial population from B); E) Study and optimization of the critical fermentation parameters, to optimize the bio transformation; F) Study and optimization of the methods for the conservation of the high-productivity cultures, guarantee stable, homogeneous inocula for productive applications on the industrial scale; G) Scale-up of the process in fermenter, in batch, fed-batch and continuous processes; H) Working up and optimization of the methods for the downstream processing and for the recovery of the product.
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