| 摘要 |
PROBLEM TO BE SOLVED: To obtain koji (melt) for production of fermented food, fermented beverage or the like, hardly causing pollution due to microorganisms by using the cereals obtained by immersing cereals into water in the presence of lactobacillus having niacin-producing ability and denaturing the cereals according to an ordinary method and using the product as a koji substrate. SOLUTION: Cereals such as de-fatted soybeans are added to the water to which a lactobacillus suspension prepared by inoculating the lactobacillus having niacin-producing ability (e.g. Lactococcus lactis ATCC11454) into a medium and culturing the lactobacillus is added, and the cereals are immersed in the solution at 15-35 deg.C for 10-50 hr and excess water is filtered and the immersed cereals are boiled and cooled to <=40 deg.C and mixed with cereals such as wheat baked and crushed according to an ordinary method. The prepared denatured cereals are used as a koji substrate to readily provide the objective koji useful for production of high-quality fermented food, fermented beverage, or the like, not causing pollution due to microorganisms and excellent in quality.
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