| 摘要 |
PURPOSE: Provided is a process for producing monoclonal antibody in large quantities, which specifically binds with T-2 toxin and can be effectively used to detection of the toxin produced by fungi contamination in cereals or livestock feeds. CONSTITUTION: A process of antibody production comprises: producing T-2 hemisuccinate(T-2HS) conjugate then T-2HS-BSA or T-2HS-HRP is produced chemically by cross-linking T-2HS with bovine serum albumin (BSA) or horse radish peroxidase(HRP), injecting the toxin-protein conjugate into a mouse, booting the antibody production by a second injection, collecting immune cells from spleen of the booster mouse, fusing the immune cells with SP2/O-Ag 14 mouse myeloma cells to produce hybridoma, screening hybridoma cell lines producing monoclonal antibody against T-2 toxin, reintroducing the selected hybridoma cells into abdominal cavity of Balb/c mouse for large production of antibody, collecting the abdominal dropsy, filter-harvesting the antibodies. T-2 toxin within cereals or feeds can be easily analyzed quantitatively or qualitatively using this monoclonal antibody by enzyme-linked immunosorbent assay (ELISA).
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