alpha 1-6 fucosyltransferase

摘要

PCT No. PCT/JP97/00171 Sec. 371 Date Jan. 7, 1998 Sec. 102(e) Date Jan. 7, 1998 PCT Filed Jan. 23, 1997 PCT Pub. No. WO97/27303 PCT Pub. Date Jul. 31, 1997Porcine- or human-derived alpha 1-6 fucosyltransferases having the following action: action: transferring fucose from guanosine adiphosphate-fucose to the hydroxy group at 6-position of GlcNAc closest to R of a receptor (GlcNAc beta 1-2Man alpha 1-6)(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc beta 1-2Man alpha 1-6)-(GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant alpha 1-6 fucosyltransferase, by culturing the transformant.